雌酮、17β－雌二醇与17α－乙炔基雌二醇在污水样中的稳定性研究

Stability tests of oestrone,17β-oestradiol and 17α-ethinyl oestradiol in sewage samples

雌酮(E1)、雌二醇(E2)和炔雌醇(EE2)在ng.L-1的浓度水平即可干扰生物的内分泌系统,在英国即将被限制排放。论文研究了在三种水样保存方式下污水厂进水和尾水中E1,E2和EE2浓度的变化。研究显示:(A)在4℃,避光保存,三种雌激素在进水样中降解率为22%、34%和4%;在尾水样中则分别为14%、26%和2%。(B)在4℃避光及pH=2(英国环保署常规有机污染物保存方法)保存条件下,E1、E2和EE2在2天内在进水样中的损失率为8%、20%和4%;在尾水样中的损失率分别为4%、17%和1%。(C)在水样中加入Cu(NO3)2抑制剂至250mg.L-1并用HCl调节至pH=3在低于10℃避光保存,可保证E1、E2和EE2在15天内损失<3%。采用方式C保存水样有利于提高分析检测E1、E2和EE2的准确度和重现性。

Oestrone （ E1 ）, 17β-oestradiol （E2） and 17α-ethinyl oestradiol （ EE2 ） are the most concerned endocrine disrupting chemicals to he regulated by envirenmental quality standards in the UK. They are present in sewage at an ng · L6-1 level. Concentrations of El, E2 and EE2 in sewage samples preserved in three different ways are analysed in a time spelL Only preserved in the dark at 4℃ （Method A） ,22% and 34% loss are seen in the STW influent sample,and 14% and 26% loss are observed for E1 and E2 in the STW effluent sample. 8% E1 and 20% E2 in the STW influent as weft as 4% of E1 and 17% of E2 in the STW effluent are lost in 2 days when preserved in the dark at 4℃ with pH = 2 （Method B） ,which is recommended for organic analytes hy the UK Environmental Agency （EA） standard method. The loss of EE2 in the above two preserving methods were less than 2% in 10 days. It is recommended to spike samples in situ with 0. 25mg Cu （NO3）2 and HC1 until pH =3 for each 1 L sample and keep in the dark at 10℃ （ Method C）. This has showed less than 3% E1 ,E2 and EE2 loss within 15 days in both influent and diluent samples.