比色法测定蒺藜中多糖的含量

Determination of Polysaccharide in Tribulus terrestris L. by Colorimetry

用苯酚－硫酸比色法测定了蒺藜（全草、果实、根）中多糖的含量。测定波长４９０ｎｍ，多糖换算因子ｆ＝３．０１；在１０～１００μｇ／ｍｌ范围内吸收度与被测物含量具有良好的线性关系，ｒ＝０．９９９５；多糖的平均回收率为９８．９０％，ＲＳＤ＝２．２３％（ｎ＝５）。测定结果表明，１０月份的全草中多糖含量最高；炮制后的果实多糖含量明显较低。

The contents of polysaccharide in Tribulus terrestris L., including whole plant, fruit and root were measured by phenol H 2SO 4 colorimetry. Wavelength for measurement was 490 nm and converting factor of polysaccharide was 3.01; Good linearity ranged from 10￣100 μg/ml, r =0.9995; Average recovery rate of polysaccharide was 98.90% with 2.23% of RSD (n =5). The results showed the highest content of polysaccharide was in the whole plant collected in Oct.; The content of polysaccharide in the processed fruit was obviously lower.