高效液相色谱法测定混剂中甜菜安和甜菜宁

Determination of Phenmedipham and Desmedipham in mixture by HPLC

本文介绍了反相液相色谱法测定混剂中甜菜安和甜菜宁,采用二极管阵列检测器和C18色谱柱的高效液相色谱仪,以甲醇︰纯水(60︰40)做为流动相,在检测波长为235nm、流速为1.0mL/min条件下,甜菜安和甜菜宁与杂质能较好的分离。该方法甜菜安的平均回收率为99.68%,变异系数0.11%;甜菜宁的平均回收率为99.74%,变异系数0.15%。

Phenmedipham and Desmedipham is using to contorl one-year old weeds with wide leafs in suger beet fields. In the article, We applied HPLC with diode array detector （DAD） at 235nm for the analysis of Phenmedipham and Desmedipham. The method involves reversed- phase separation of the components on C-18 bonded silica. The sample was dissolved in methanol, Phenmedipham and Desmedipham in the sample is separated and determined using a 250mm×3.9mm （i.d） stainless steel column packed with ODS, with methanol-water （60＋40, V/V） as the mobile phase. Recovery rates ranged form 99.29-100.14%.The coefficient of variation for the method were 0.11% and 0.15%. The procedure was highly selective and reproducible and be successfully used in determining contents of Phenmedipham and Desmedipham on macro leves.