镧示踪法观察大鼠颅脑损伤后血脑屏障形态学变化

Observation of Morphological Changes of Blood brain Barrier After Brain Injury in Rats with Lanthanum

目的：颅脑损伤后血脑屏障（ｂｌｏｏｄ－ｂｒａｉｎｂａｒｒｉｅｒ，ＢＢＢ）通透性的变化，是导致外伤性脑水肿的最直接的因素．研究脑ＢＢＢ改变对探讨颅脑损伤脑水肿发生发展过程有重要意义．方法：本实验采用镧示踪法观察大鼠脑损伤后ＢＢＢ超微结构的变化，同时定量检测了伤区脑组织脑水肿发展过程．结果：研究发现伤后１０分钟组，已可见ＢＢＢ开放，伤后２４～４８小时组渗出最为严重，伤后７天组ＢＢＢ功能尚未完全恢复．颅脑损伤后伤区脑组织伊文氏蓝（ｅｖａｎｓｂｌｕｅ，ＥＢ）含量及伤区脑组织含水量的变化过程与ＢＢＢ的改变相一致．结论：对颅脑损伤后脑水肿的治疗应从早期开始，并持续１周以上．

Aim: To study the changes of permeability of blood brain barrier (BBB) which directly cause brain edema after brain injury. It is very important to explain the development of brain edema. Methods: We observed the changes of the ultrastructure of BBB with lanthanum and the water content of brain after brain injury in rats. Results: It was found that BBB opened at 10min and reached its peak between 24-48 hours and the peak lasted till 168 hours after brain injury. The water and evans blue (EB) content had the same changes as that of BBB. Conclusion: The changes of permeability of blood brain barrier start earlier and become profound gradually after brain injury. So the treatment of brain injury should begin earlier and last 7 days at least.