摘要
目的:探讨不同剂量腺苷与AMP579对不同类型冠心病(CHD)患者中性粒细胞表面CD11b/CD18表达的影响。方法:选择经临床症状、心电图改变及心肌酶学确诊的CHD患者及正常健康者采血,患者血液分别给予不同剂量的腺苷与AMP579进行干预,免疫荧光标记后用流式细胞仪分别检测正常人及患者用药前后中性粒细胞CD11b/CD18的表达值。结果:CHD患者的中性粒细胞CD11b/CD18的表达较正常人明显升高(P<0.01);腺苷在10,30,100μmol能有效降低CHD患者的中性粒细胞CD11b/CD18的表达(P<0.05),但30,100μmol组之间差异无显著性(P>0.05);AMP579组中,患者中性粒细胞CD11b/CD18的表达均未见明显降低,各组较对照组差异均无统计学意义(P>0.05)。结论:CHD的发生与CD11b/CD18表达升高有关;腺苷可通过降低CD11b/CD18的表达而降低白细胞与内皮细胞之间的黏附性;而AMP579在1,10,30μmol尚不能降低CHD患者CD11b/CD18的表达。
Objective ：To explore the effect of the different doses AMP579 and adenosine to the expression of adhesion molecules CD11b/CD18 on neutrophils in different types of patients with coronary heart disease（CHD）. Methods ：The patients and normal healthy individuals were drew blood ,patients blood were given different doses of adenosine and AMP579 to interval,the expression of CD11b/CD18 on neutrophils of normal people and patient were measured by direct immunofluorescence techniques associated with flow cytomerey. Results ：Expression of CD11b/CD18 on neutrophils in the CHD patients were markedly higher than those in normal cases （P〈0. 01）. Adenosine can reduced expression of CD11 b/CD18 in CHD patients on neutrophils in 10,30, 100 μmol （P 〈 0. 05 ）, but expression of CDllb/CD18 in 30 μmol and in 100 μmol were no significantly different（P〈0.05）. In AMP579 groups,the expression of CD11b/CD18 in CHD patients on neutrophils were no decreased significantly,there were no statistical significant （P〈0. 05）. Conclusion ：The incidence of coronary heart disease related with the increasing of CD11b/CD18 expression,adenosine can reduce CD11b/CD18 expression to reduce the adhesion of white blood cell and endothelial cell. AMP579 can＇t reduce the expression of CD11b/CD18 on neutrophils of CHD patients in 1,10,30 μmol.
出处
《临床医药实践》
2008年第6期405-408,共4页
Proceeding of Clinical Medicine
