热休克蛋白70在大鼠急性肺损伤中的表达

Expression of HSP70 in acute lung injury

目的:探讨急性肺损伤(ALI)大鼠肺组织热休克蛋白70(HSP70)的表达。方法:雌性Wistar大鼠36只,随机分为两组:急性肺损伤组(A组)、对照组(B组),大鼠尾静脉注射脂多糖(LPS)复制ALI模型。所有的动物于注射LPS后2,4,6 h处死,测定肺组织HSP70的表达、超氧化物歧化酶(SOD)活性及丙二醛(MDA)的含量。结果:A组同B组相比,各时间点肺组织HSP70表达均增多,以2,6 h升高明显(P<0.01),MDA含量增高(P<0.05),SOD活性明显降低(P<0.05)。结论:HSP70在ALI后的表达增多可能与其参与LPS所致肺损伤后的组织保护有关。

Objective：To expolre the expression of heat shock protein（HSP70） in acute lung injury（ALI）. Methods ：Thirty-six female wistar rats were randomly divided into two groups ：ALI group（group A）,control group（group B）. The rat model of ALI was established with injecting lipopolysaccharide（LPS）through caudal vein. All the rats were killed at 2,4,6 h afer injecting LPS. Expression of HSP70 of the lung tissue the activity of SOD and the contents of MDA of the lung tissud were determined. Results ：In the A group ,expression of HSP70 in the lung tissue increased in every time points than the group B. The 2 , 6 h are obviously （ P 〈 0. 0 1 ） , the activity of SOD became lower （ P 〈0. 05） and the contents of MDA increased（P〈0.05）. Conclusion ：The results suggests that HSP70 may play a role in the protection of ALI induced by LPS.