新型材料PLLA-脱细胞软骨粒支架的制备及性状研究

The preparation and the character research of new material PLLAacellular cartilaginous matrix

目的:以聚左旋乳酸(PLLA)和猪的脱细胞软骨粒混合物为材料制备一种新型的支架,即PLLA-脱细胞软骨粒支架,并对其性状进行研究,从而为软骨组织工程提供一种新的支架材料。方法:利用热相致分法(T IPS法)制备PLLA与脱细胞软骨粒在不同混合比例、不同温度下的聚合物支架,并对各种支架的孔径大小、孔隙率、水结合力、生物力学性状、降解率等进行探讨。结果:PLLA-脱细胞软骨粒支架具有与天然软骨基质相近的成分与结构,其各种性状基本符合软骨支架的要求。结论:PLLA-脱细胞软骨粒支架可作为软骨组织工程良好的细胞外基质材料。

Objective：Using the mixture of poly（L-lactide） and the pig acellular cartilaginous matrix to prepare a new scaffold as the material was PLLA-acellular cartilaginous scaffold ,and making the research to its characters ,thus provides a new scaffold for the cartilage tissue engineering. Methods ：A three-dimensional polymer scafold was developed by mixing PLLA with acellular cartilaginous matrix using a thermally induced phase separation technique （TIPS） under the different proportion of mixture and the, different temperature. Each kind of scaffold pare size ,porosity,the water-binding capacity ,the biological mechanics character ,the biology degradability and so on were disgussed. Resuls：The PLLA-acellular cartilaginous sandwich composite was obtained,mimicking the natural cartilage both in composition and microstructur. Its various characteristics have basically conformed to the requirement of cartilage scaffold material. Conclusion ：The novel three dimensional PLLA-acellular cartilaginous matrix scaffold is likely to be applied in the cartilage tissue engineering.