芝麻素对H_(22)荷瘤小鼠的抑瘤作用及其机制

Inhibitory effect of sesamin on tumor growth and mechanism in H_(22) hepatoma-bearing mice

目的观察芝麻提取物芝麻素对H22荷瘤小鼠的抑瘤作用,并探讨其作用机制。方法将移植H22肝癌瘤株的64只昆明种小鼠随机分成4组:模型组、环磷酰胺组、芝麻素高浓度组及芝麻素低浓度组,连续用药10d。比较各组抑瘤率、胸腺指数、脾指数及增殖细胞核抗原(PCNA)表达水平。结果低浓度的芝麻素在体内对H22肿瘤细胞的生长有明显的抑制作用,使动物的免疫器官重量增加,并对肿瘤组织PCNA的表达有显著的抑制作用。结论芝麻素抑制H22细胞的机制可能是通过促进宿主免疫功能、抑制肿瘤细胞内PCNA表达来实现的。

Objective To observe the antitumor effect of sesamin on H22 hepatoma-bearing mice and explore its mechanism. Methods 64 mice embedded with H22 hepatoma were equally divided into 4 groups： model group （saline）, cyclophosphamide group （0.2 g/L）, high concentration sesamin group （15 g/L）, low concentration sesamin group （1.5 g/L）. Every group received corresponding treatments for 10 days. The inhibition rate of tumor, thymus, spleen index and expres- sion intensity of proliferating cell nuclear antigen in the tumor tissue of the 4 groups were compared. Results Low concentration sesamin could significantly inhibit growth of H22 hepatocareinoma cells in vivo, promot the immunity function and reduced the expression of PCNA in the tumors. Conclusion The inhibitory mechanism of sesamin on H22 hepatocareinoma cells may be realized by promoting the immunity function and inhibiting the expression of PCNA.