脑缺血再灌注后依达拉奉联合黄芪对大鼠神经元的保护作用及机制

Mechanism and protective effect of edaravone combined with astragalus on the nerve cell after ischemia and reperfusion in rats

目的探讨脑缺血再灌注后依达拉奉联合黄芪对大鼠神经元的保护作用及机制。方法将SD大鼠随机分为假手术组、缺血对照组、黄芪组、依达拉奉组、依达拉奉与黄芪联合组及溶剂组。采用焦油紫染色、免疫印迹检测大鼠缺血再灌注后海马神经元损伤和脑组织JNK1/2、bad(ser 128)和c-Jun的磷酸化情况。结果各给药组与缺血再灌注组和溶剂组相比,海马神经元损伤减轻,脑组织JNK1/2、c-Jun和bad(ser 128)磷酸化降低,其中联合组效果更明显(P均<0.05)。结论依达拉奉、黄芪两药联用比单用更能减少海马神经元损伤及脑组织JNK1/2、c-Jun、bad(ser 128)的磷酸化程度。

Objective To investigate the mechanism and protective effect of edaravone combined with astragalus on the nerve cells after ischemia and reperfusion in rats. Methods SD rats were divided randomly into 6 groups ： shamoperated group, cerebral ischemia control group , edaravone group, astragalus group, astragalus ＋ edaravone group and solvent group. Brain injury in hippocampus of rats and change of phosphorylation of JNK1/2, c-Jun and bad（ ser 128） were detected by cresly violetstrained or immunoblot assay. Results Comparing with ischemia group and solvent group,the brain injury and phosphorylation of JNK1/2, c-Jun and bad（ ser 128） of administration groups decreased, and more significantly of edar- avone combined with astragalus. Conclusions Combination of edaravone and astragalus can decreae the brain injury and phosphorylation of JNK1/2 ,c-Jun and bad（ set 128） more significantly than single medication.