缺血性脑血管病患者趋化因子受体CX3CR1基因V249 I多态性的研究

Research on V249I polymorphisms of chemokine receptor CX3CR1 gene in patients with ischemic cerebrovascular disease

目的探讨缺血性脑血管病(ICVD)患者趋化因子受体CX3CR1基因V249 I的多态性及其频率。方法采用聚合酶链反应和限制性片段长度多态性方法检测ICVD患者(脑梗死、腔隙性脑梗死、短暂性脑缺血发作)及健康对照者的CX3CR1基因V249 I的多态性及频率。结果对照组CX3CR1基因V249 I只有VV和VI基因型,ICVD组有VV、VI和Ⅱ3种基因型;ICVD组I等位基因频率明显高于对照组(P<0.01);CX3CR1基因型及I等位基因频率在不同类型ICVD患者之间无统计学差异。结论CX3CR1基因V249 I多态性可能与ICVD有关。

Objective To investigate chemokine receptor CX3CR1 gene V249I polymorphisms and its frequency in patients with ischemic cerebrovascular disease （ICVD） . Methods The CX3CR1 gene V249I polymorphisms and frequency of patients with ICVD（ cerebral infarction, lacunar infarction, transient ischemic attack） and healthy controls were analyzed by multiplex polymerase chain reaction and restriction fragment length polymorphism. Results There were VV and VI genetypes of CX3CR1 gene V249I in control group,and VV,VI and Ⅱ genetypes in ICVD group. The frequency of I249 allele was significantly higher in ICVD group than that in control group（P 〈0.01 ）. No significant differences were observed in ICVD subgroups in genetypes and I allele distribution. Conclusions CX3CR1 gene V249I polymorphism may be associated with ICVD.