CYP1A1及GSTM1基因多态性对肺癌发病的影响

Influence of genetic polymorlhism of CYP1A1 gene and GSTM1 gene on lung cancer

目的探讨代谢活化酶细胞色素P4501A1(CYP1A1)及谷胱甘肽硫转移酶M1(GSTM1)基因多态性和环境暴露与肺癌易感性的关系。方法用聚合酶链反应—限制性片段长度多态性技术测定158例肺癌患者和455例对照者的CYP1A1及GSTM1基因多态性。结果与对照组比较,肺癌组吸烟、粉尘接触频率明显升高,而摄入蔬菜水果及消毒水频率明显降低(P均<0.01);两组CYP1A1与GSTM1基因各类型分布无统计学差异;CYP1A1突变型基因及GSTM1缺陷型与吸烟有协同致肺癌作用。结论吸烟、接触粉尘均增加肺癌发生率,而摄入蔬菜水果及消毒水降低其危险性;吸烟可增加CYP1A1基因突变型或GSTM1基因缺陷型个体肺癌发生的危险性。

Objective To explore the relations among the polymorphism of CYP1A1 gene and GSTM1 gene, environmental exposure and the susceptibility to lung cancer. Methods The polymorphisms of the CYP1A1 gene and GSTM1 gene were detected by the method of polymerase chain reaction and restriction fragment length polyorphism. Results Compared with control group , the frequencies of smoking and dust exposure were higher ,While the frequencies of eating vegetables, fruits and drinking sanitized water were lower in lung cancer（P all 〈0.01 ）. There was no statistical difference in the distribution of each genotype of CYP1A1 and GSTM1 in the two groups. There was a synergistic interaction to lung cancer among CYP1 A1 mutant genotype, GSTM1 null genotype and tobacco exposure. Conclusions Smoking and dust exposure significantly increase the risk of lung cancer, intaking vegetables, fruits and sanitized water frequently decrease the risk of lung cancer, there is an elevated risk of lung cancer in CYP1A1 mutant genotype with exposure to tobacco, as well as GSTM1 null genotype.