近皮层大鼠C6胶质瘤模型的建立研究

Research on a near cortex glioma model

目的:建立一种便于开骨窗及肿瘤切除的近皮层大鼠C6胶质瘤模型。方法:立体定向下将C6细胞接种于大鼠脑皮层、白质交界处,建立近皮层荷瘤鼠模型。取7只模型鼠,分别于接种的第7、11、15天行头颅MR I检查,了解肿瘤的生长情况。行病理HE切片观察、流式细胞DNA细胞周期分析及GFAP免疫组织化学检测,了解肿瘤的生长特性和病理特征。5只荷瘤鼠及2只正常大鼠经尾静脉注入5-ALA后,取肿瘤组织及脑组织制成细胞悬液行荧光分光光度分析,探讨血脑屏障的破坏情况。取29只荷瘤大鼠,分成开骨窗组、开骨窗加取瘤组及对照组,观察生存期。结果:用本法建立近皮层C6胶质瘤模型54只,52只成功建立模型,成功率96.3%。病理、流式细胞、免疫组织化学显示大鼠C6胶质瘤生长特性和病理特征与人脑胶质瘤相似;对照组、开骨窗组及开骨窗加肿瘤切除组生存期分别为(32.000 0±8.981 5)、(41.888 9±6.461 8)、(59.400 0±14.416 0)d。行组间单向方差分析,生存期比较有统计学差异(P<0.05)。结论:该方法建立的胶质瘤模型稳定,生长特性和病理特征与人脑胶质瘤相似,便于开骨窗及切除肿瘤,是进行胶质瘤研究的较好模型。

Objective To establish a near cortex glioma model as a laboratory foundation for research in human brain glioma. Methods 54 male SD rats were inoculated with C6 cell suspension by stereotaxic technique and received MRI, HE and GFAP immunohistochemistry. 29 rats were divided into 3 groups which are craniectomy group, craniectomy plus resection group and control group to see the survival of rats. Results The model established by this method had the similar characters to human glioma. The survival time of rats between these there groups has significant difference being（32.0000±8.981 5）,（41.888 9±6.461 8）and（59.4000±14.416 0） days. Conclusion The model established is suitable for the study of brain neoplasm.