电针对Walker-256模型大鼠肿瘤生长和免疫功能的影响

Effects of electroacupuncture on tumor growth and immune function in the Walker-256 model rat

目的:研究针刺治疗肿瘤的效应及作用机制。方法:采用Walker-256细胞株复制种植性Wistar大鼠肝癌、胃癌、皮下瘤模型,造模第3d,3种模型大鼠分别随机分为模型组和电针组。电针组针刺"足三里""合谷""三阴交",予电针治疗,每天1次,留针20min,连续治疗15d。检测各组大鼠肿瘤的体积和肿瘤抑制率及各组大鼠外周血中体液免疫(血清免疫球蛋白IgG、IgM、IgA和补体C3、C4)以及细胞免疫(T淋巴细胞亚群CD4+、CD8+和CD4+/CD8+比值)。结果:电针组肿瘤体积显著低于其模型组(P<0.01)。针刺组外周血IgG、IgM、C3含量较其模型组显著增高(P<0.05,P<0.01),IgA和C4含量较模型组增高不明显(P>0.05)。电针组外周血CD4+和CD4+/CD8+比值均显著高于其模型组(P<0.05,P<0.01)。结论:电针"足三里""合谷""三阴交"能提高大鼠Walker-256肝癌、胃癌、皮下瘤的免疫功能,抑制其肿瘤的生长。

Objective To study the effect of acupuncture on tumor and its mechanism. Methods Liver cancer, gastric cancer and hypodermic tumor rat models were made by implantation of replicated Walker-256 cell strain. The 3 model rats were respectively divided into two groups at random, a model＇control group and an electroacupuncture group. The clectroacupuncture groups were treated with electroacupuncture （EA） at ＂Zusanli＂ （ST 36）, ＂Hegu＂ （LI 4） and ＂San-yinjiao＂ （SP 6）, once each day, 15 min one session, for 15 days. The gross tumor volume and the tumor inhibitory rate, andthe levels of humoral immunity index, including serum IgG, IgM, IgA and Ca, C4 , and the levels of cellular immunity index, including CD4^＋ , CD8^＋ and CD4^＋/CD8^＋ in the peripheral blood in each group were detected. Results The gross tumor volumes in the EA groups were significantly smaller than those in the model control group （P〈0.01）. The contents of IgG, IgM and C3 in the EA groups increased significantly compared with those in the model control group （P〈20.05 or P〈20.01）. The contents of IgA and C4 in the EA groups did not significantly change （P〉0.05）. The content of CD4^＋ and CD4^＋/CD8^＋ in the EA groups are significantly higher than those in the model control group （P〈0.05 or P〈0.01）. Conclusion Acupuncture at ＂Zusanli＂ （ST 36）, ＂ Hegu＂ （LI 4） and ＂Sanyinjiao＂ （SP 6） can increase immune function and inhibit tumor growth in Walker-256 liver cancer, gastric can-cer and hypodermic tumor rats.