IL-12基因转染B细胞的抗原提呈功能研究

IL-12 Retroviral Expression Vector pL35P40SN Transfected B Cell as a Adjuvant for Tumor Vaccine

目的 以IL-12双亚基共表达载体pL35P40SN转染B细胞,并检测其对Th细胞发育分化和CTL功能的影响。方法 尼龙毛法分离T,B细胞,以pL35P40SN转染B细胞,并以ELISA检测其IL-12的分泌,以IFN-γ的合成分泌检测其对Th1细胞发育分化的影响,以^3H-TdR法检测其对CTL特异杀伤HepG2细胞功能的影响。结果 以pL35P40SN转染B细胞后,pL35P40SN基因转染的B细胞IL-12分泌量、Th应答细胞上清IFN-γ分泌量和CTL的杀伤功能显著高于pLXPXSN和未转染的细胞。结论 pL35P40SN转染B细胞后,可刺激Th1细胞发育分化和CTL的特异性杀伤功能。

Objective To transfect B cell with recombinant human IL-12 retroviral expression vector pL35P40SN, and to identify its influence on Th cell differentiation and CTL function. Methods We transfect B cell with pL35P40SN, detect IL-12 production in the cells supernatant with ELISA method,detect the transfected B cellg influence on Th cell differentiation and CTL function, with IFN-γ production and 3 H-TdR method respectively. Results When transfected B cell with recombinant human IL-12 retroviral expression vector pL35P40SN,There were more IL-12 produc- tion in the cells supernatant based on ELISA detection,there were more IFN-γ production in the Th cells supernatant, CTL function were more stronger then the pLXPXSN transfected B cell. Conclusion pL35P40SN transfected B cell can promote Th1 cell differentiation and CTL function.