摘要
目的鉴定鸡源副黏病毒F48E9株的自然宿主细胞膜受体。方法采用蛋白膜提取试剂盒提取鸡胚成纤维细胞膜蛋白,用改进的间接ELISA方法检测鸡源副黏病毒分离株F48E9与细胞膜的结合活性,利用病毒铺覆蛋白印迹技术(VOPBA)鉴定新城疫病毒(NDV)的自然宿主细胞膜受体。结果NDV与鸡胚成纤维细胞膜有很强的结合力,在转印鸡胚成纤维细胞膜蛋白的PVDF膜上有几条明显的疑似受体条带,相对分子质量介于35000～60000之间。结论初步鉴定了NDV自然宿主细胞膜的受体,其疑似受体蛋白的性质及在新城疫病毒致病中的作用尚有待进一步研究。
Objective To identify the receptor of paramyxovirus F4sE9 strain from chick origin on natural host cell membrane. Methods Extract the membrane protein of chick embryo fibroblast （CEF） by using membrane extraction kit. Determine the binding activity of isolated paramyxovirus F48E9 strain to cell membrane by a modified indirect ELISA. Identify the Newcastle disease virus （NDV） receptor on host cell membrane by virus overlay protein blot assay （VOPBA）. Results Several suspected receptor bands with relative molecular masses of 35 000 to 60 000 were observed on the PVDF membrane blotted with CEF, which showed specific binding to NDV. Conclusion The receptor of NDV on natural host cell membrane was preliminarily identified. The properties of the suspected receptors as well as their roles in pathology of NDV were to be further studied.
出处
《中国生物制品学杂志》
CAS
CSCD
2008年第8期651-653,共3页
Chinese Journal of Biologicals
基金
国家自然科学基金资助项目(30571375)
关键词
新城疫病毒
自然宿主
细胞膜受体
病毒铺覆蛋白印迹技术
Newcastle disease virus （NDV）
Natural host
Membrane receptor
Virus overlay protein blot assay （VOPBA）
