哺乳期大鼠大量摄入酒精对子代成年期胰岛素敏感性的影响

Effect of Maternal Ethanol Heavy Consumption During Lactation on Sensitivity to Insulin of Rat Offspring in Adulthood

目的观察哺乳期大鼠大量摄入酒精对子代成年期胰岛素敏感性的影响。方法将Wistar雌鼠哺乳期酒精灌胃4g/kg·d,对照组给予等容积蒸馏水。子代出生后,每周测量体重1次,断乳后1周开始测量进食量;对第16周龄子代雄性鼠进行静脉葡萄糖耐量试验;Western blot检测骨骼肌细胞膜葡萄糖转运蛋白4(GLUT4)含量;酶学法测定血浆和骨骼肌甘油三酯(TG)含量。结果哺乳期大鼠摄入酒精对后代生长发育无明显影响。与对照组比较,哺乳期大鼠摄入酒精的子代鼠16周龄时,葡萄糖耐量降低,血糖曲线下面积增加,胰岛素敏感指数(SI)、处置指数(DI)及葡萄糖耐量指数(KG)均明显降低,但葡萄糖效应指数(SG)与对照组比较,差异无显著意义,葡萄糖刺激的胰岛素快速反应分泌总量差异亦无显著意义;哺乳期大鼠摄入酒精的子代鼠,骨骼肌细胞膜GLUT4含量、血浆及骨骼肌TG含量与对照组比较,差异均无显著意义。结论哺乳期大鼠大量摄入酒精可引起后代成年期发生胰岛素抵抗,其发生机制与骨骼肌GLUT4转位及TG含量无关。

Objective To observe the effect of maternal ethanol heavy consumption during lactation on the sensitivity to in- sulin of rat offspring in adulthood. Methods Female Wistar were given ethanol 4g / kg·d by gavage throughout the lactation period, using distilled water as control. The male offspring after birth were measured for bodyweight once a week until the age of 16 weeks, during which the diet taken after weaning was observed from the 4th week. Perform intravenous glucose tolerance test on the offspring at age of 16 weeks. Determine the skeletal muscle cell membrane glucose transporter 4（ GLUT4 ） by Western blot, and the triglyceride （TG） contents in skeletal muscle and plasma by enzymological method. Results The maternal ethanol heavy consumption during lactation showed no significant effect on growth of offspring. Compared with those of control, the glucose tolerance level, insulin sensitivity index（S1）, disposition index（ DI ） and glucose tolerance index （KG） of 16-week-old offspring of rats with ethanol heavy consumption during lactation decreased significantly, while the area under blood glucose curve increased significantly. However, the glucose effectiveness （SG）, acute insulin response level to glucose, skeletal muscle cell membrane glucose transporter 4 （GLUT4） as well as triglyceride （TG） contents in skeletal muscle and plasma showed no significant difference from those of control. Conclusion Maternal ethanol heavy consumption during lactation induced insulin resistance in rat offspring, which was unassociated with GLTU4 and TG level in muscle tissue.