氧化型低密度脂蛋白的制备及其对血管平滑肌细胞增殖能力的影响

Preparation of Oxidative Low Density Lipoprotein and Its Effect on Proliferation of Vascular Smooth Muscle Cells

目的制备氧化型低密度脂蛋白(ox-LDL),并检测其对血管平滑肌细胞(SMC)增殖能力的影响。方法肝素沉淀法提取LDL,Cu2+氧化法制备ox-LDL,MTT法测定其对Wistar大鼠血管SMC增殖能力的影响。结果LDL完全被氧化成ox-LDL SDS-PAGE显示,LDL相对分子质量降低;200mg/Lox-LDL可刺激体外培养的SMC增殖能力明显增强;ox-LDL浓度不低于600mg/L时,SMC增殖能力明显下降。结论成功制备了ox-LDL,一定浓度的ox-LDL可促进SMCs的增殖。

Objective To prepare oxidative low density lipoprotein （ox-LDL） and explore its effect on the proliferation of vascular smooth muscle cells （SMCs）. Methods Extract LDL by heparin precipitation and prepare ox-LDL by copper ion oxidation. Determine the proliferation level of SMCs by MTT method. Results LDL was oxidized completely, and SDS-PAGE showed significant decrease of relative molecular mass of the obtained ox-LDL. The proliferation level of SMCs stimulated with 200 mg/L ox-LDL increased significantly. However, when the concentration of ox-LDL was not less than 600 mg/L, the proliferation level of SMCs decreased significantly. Conclusion ox-LDL was successfully prepared. The prepared ox-LDL at a certain concentration stimulated the proliferation of vascular SMCs significantly.