摘要
目的制备氧化型低密度脂蛋白(ox-LDL),并检测其对血管平滑肌细胞(SMC)增殖能力的影响。方法肝素沉淀法提取LDL,Cu2+氧化法制备ox-LDL,MTT法测定其对Wistar大鼠血管SMC增殖能力的影响。结果LDL完全被氧化成ox-LDL SDS-PAGE显示,LDL相对分子质量降低;200mg/Lox-LDL可刺激体外培养的SMC增殖能力明显增强;ox-LDL浓度不低于600mg/L时,SMC增殖能力明显下降。结论成功制备了ox-LDL,一定浓度的ox-LDL可促进SMCs的增殖。
Objective To prepare oxidative low density lipoprotein (ox-LDL) and explore its effect on the proliferation of vascular smooth muscle cells (SMCs). Methods Extract LDL by heparin precipitation and prepare ox-LDL by copper ion oxidation. Determine the proliferation level of SMCs by MTT method. Results LDL was oxidized completely, and SDS-PAGE showed significant decrease of relative molecular mass of the obtained ox-LDL. The proliferation level of SMCs stimulated with 200 mg/L ox-LDL increased significantly. However, when the concentration of ox-LDL was not less than 600 mg/L, the proliferation level of SMCs decreased significantly. Conclusion ox-LDL was successfully prepared. The prepared ox-LDL at a certain concentration stimulated the proliferation of vascular SMCs significantly.
出处
《中国生物制品学杂志》
CAS
CSCD
2008年第8期725-726,731,共3页
Chinese Journal of Biologicals
关键词
氧化型低密度脂蛋白
肝素
血管平滑肌细胞
增殖
Oxidative low density lipoprotein (ox-LDL)
Heparin
Vascular smooth muscle cells
Proliferation