喹诺酮类药物与铜锌超氧化物歧化酶的相互作用及其对酶活性的影响

Interaction between Copper,Zinc Superoxide Dismutase and Quinolones

利用紫外-可见吸收光谱和荧光光谱,在pH=7.4的生理条件下,研究了喹诺酮类药物(依诺沙星、环丙沙星和诺氟沙星)与铜锌超氧化物歧化酶(CuZnSOD)的相互作用。喹诺酮类药物能使CuZnSOD的荧光发生猝灭,其猝灭机理为静态猝灭。通过实验计算了不同温度下喹诺酮类药物与CuZnSOD的结合常数和结合点数。依据Frster非辐射能量转移理论,得到供体与受体间的距离。根据热力学参数确定了它们之间的主要作用力类型是静电引力。进一步证实了活体动物兔子注射环丙沙星后,血液中铜锌超氧化物歧化酶活性显著降低。

The interactions between quinolones （norfloxacin, ciprofloxacin, enoxacine） and copper, zinc superoxide dismutase （CuZnSOD） were investigated using absorption and fluorescence spectrometry. Quinolones can make CuZnSOD fluorescence quenching. The quenching mechanism is static quenching. The binding constants of quinolones with CuZnSOD were obtained at various temperatures. According to the Forster nonradiative energy transfer theory, the distance of donators and acceptors can be obtained. The main acting force between them is electrostatic gravitation. Quinolones can decrease the activity of CuZnSOD and the activity of CuZnSOD in rabbits blood being injected with ciprofloxacin is also decreased obviously.