消化道肿瘤自身抗体检测

Analysis of Autoantibody in Patients with Gastrointestinal Neoplasms

目的探讨消化道肿瘤患者针对肿瘤细胞的自身抗体的存在。方法收集肠癌患者及胃癌患者术前血清96份和健康体检人群血清62份,分组后采用Western印迹分析法定性检测消化道肿瘤患者术前血清中的自身抗体。结果正常健康人群和正常肝细胞株96%(212/220)的反应条无显色带,而97%(60/62)的肠癌患者肠癌细胞株、89%(39/44)的胃癌患者胃癌细胞株反应条均出现多个阳性带。结论健康人群体内很少产生针对肿瘤细胞的自身抗体,而大部分消化道肿瘤患者体内产生针对肿瘤相关抗原的自身抗体,这为消化道肿瘤的病因,早期诊断,预后监测及分子靶向治疗等研究提供了一个很好的线索。

Objective To examine the presence of autoantibody for tumor cells in patients with gastrointestinal neoplasms. Method Serum from 62 normal subjects and 96 patients with intestinal or gastric cancer was analyzed by Western-blot for the presence of autoantibodies. Result 96% of the samples from the normal subjects were negative for the autoantibody. 97% of test bands in the healthy population and normal liver cell strains, while some positive color reactions were showed on 97% （60/62） of test bands in the intestine cancer cell line and 89%（39/44） of gastric cancer cell line. Conclusion Autoantibodies against tumor cells were rarely produced in the health population, while they existed in most of patients with gastrointestinal neoplasms. It was a good clue to the reseach of etiology, early diagnosis, prognosis monitoring and molecular targeted therapy of gastrointestinal neoplasms.