诊断用彩色多普勒超声辐照对小鼠早孕胚胎细胞超微结构的影响

Influence of diagnostic CDFI on ultrastructure of the rats embryos cell

目的：应用彩色多普勒超声辐照早孕小鼠胚胎,观察小鼠早孕胚胎细胞超微结构的变化。方法：8只孕6～7 d成年昆明种小鼠,按彩超辐照时间不同随机等分为4组,组（对照组）、组（辐照10 min组）、组（辐照20 min组）、组（辐照30 min组）,仪器采用百胜公司DU-8型彩色超声诊断仪。照射条件：腹部凸阵探头,探头频率为3.0MHz,MI=0.9 TiS=0.9。照射后24 h取小鼠胚胎标本,HE染色光镜观察组织结构,透射电镜观察超微结构变化。结果：各组光镜下观察胚胎组织结构均未见异常。照射30 min组小鼠胚胎细胞电镜检查发现染色质浓缩边集、线粒体肿胀等超微结构变化。结论：彩色多普勒超声辐照早孕小鼠可引起胚胎细胞超微结构发生改变。

Objective ：To study the influence of diagnostic CDFI on ultrastructure of the rats embryos cell. Methods ：Eight female rats that had been pregnant for 6-7 days were divided into four groups averagely and randomly： control group （group Ⅰ）was sham-irradiated ;group Ⅱ -Ⅳ were irradiated by diagnostic CDFI（H = 3.0 MHz,Tis = 0.9, MI=0. 9）for 10 min,20 min and 30 min respectively. The fetal rats＇embryos of the four groups were resected after 24 hours irradiation and HE stain was done for microscopic observation ;ultrastructural changes were observed by transmission electronmicro scope. Results ：There was no abnormal changes among group Ⅰ -Ⅳ under microscope. The ultrastructural changes of the embryos cell in group Ⅳ were observed by transmission electronmicroscope, which showed condensation of the nuclear chromatin with margination,mitochondria swelling, etc. Conclusions ：Continuous irradiation for 30 min by diagnostic CDFI on fetal rat in pregnancy may result in ultrastructural changes.