rhEPO对大鼠肝脏缺血再灌注损伤的保护作用

Protection of rhEPO on hepatic ischemic/reperfusion injury in rats

目的:探讨rhEPO对大鼠肝脏缺血再灌注损伤中的保护作用及其处理时间。方法:健康Wistar大鼠36只,随机分为对照组A、缺血前30 min预处理组B和缺血后5 min后处理组C 3组,每组12只。A组只给予夹闭肝蒂,B、C组均于皮下注射rhEPO 1 000 U/kg,三组均夹闭肝蒂45 min后恢复再灌注2 h,取血清及肝组织行AST、ALT、TNF-α、IL-1β检测及HE染色、NF-κβ的活化程度及计算肝组织凋亡指数(AI)。结果:与A组比较,B、C组肝细胞索排列较好,细胞坏死程度不明显。AST、ALT和TNF-α、IL-1β及NF-κB活化程度、AI在A组和B、C组之间均有显著差异(P<0.05),B和C组之间(除IL-1β外)也具有统计学意义(P<0.05)。结论:rhEPO对缺血再灌注损伤的肝脏具有保护作用且预处理较后处理效果好。

Objectives：To explore the protective effect of rhEPO on hepatic ischemia reperfusion injury in rats and treatment time. Methods：Thirty-six wistar rats were randomly divided into three groups, the control group A, the rhEPO pretreatment group B and postprocessing group C,n= 12 respectively. Liver pedicles were occlused in group, rats were injected rhEPO 1 000 U/kg into subcutaneously 30 minutes before ischemia in B group or 5 minutes after in C group A. After 45 minutes ischemia,then followed reperfusion. Serum aspartic acid aminotransferase（AST）and alanine aminotransferase（ALT）,tumor necrosis factor α（TNF-α） and interleukin 1 β（IL-1β）were measured and liver histo-pathology was observed by HE staining. Nuclear factor κB（NF-κB） was detected by immunohistochemistry and apoptotic index was calculated by TUNEL. Results：Compared to group A,the arrargement of liver cell--line is relatively better and the cells were damaged insignificantly in group B and C. The serum concentration of AST,ALT, TNF-α,IL-1β,NF-κB and AI in group A have difference significantly（P〈0. 05）,and group B and C had statistical significance （except IL-1β）. Conclusion：RhEPO protects the liver from ischemia reperfusion injury in rats and pretreatment is better than postprocessing.