雌二醇对实验性自身免疫性脑脊髓炎大鼠炎性细胞凋亡的研究

Estrogen may induce the apoptosis of the inflamatory cells in rats with experimental autoimmune encephalomyelitis

目的:探讨雌二醇(E2)对去卵巢后实验性自身免疫性脑脊髓炎(EAE)大鼠脑脊髓细胞凋亡的作用。方法:建立去卵巢10d后Wistar大鼠EAE模型,随机分为EAE组、E2治疗组和假手术组。E2治疗组从发病后连续3d,每日肌肉注射E2(1mg/kg)1次。在免疫后16d处死动物,取脑和脊髓用3′末端脱氧核糖核酸转移酶介导的duTP缺口末端标记(TUNEL)原位末端法检测大鼠脑脊髓凋亡细胞数。结果:与EAE对照组相比,E2治疗组和假手术组的中枢神经系统(CNS)TUNEL阳性细胞的凋亡率明显增高。结论:E2对EAE有治疗作用,其机制可能与其抑制CNS炎症细胞浸润及增加炎性细胞的凋亡有关。

Objective：To investigate the apoptosis of the inflamatory cells induced by estrogen in wistar rats with experimental allergic encephalomyelitis（EAE） which were ovariectomized.Methods：Wistar rats which were ovariectomized ten days were randomly divided into EAE group,E2 group and sham operated group.E2 group were injected with E2（1 mg/kg）once a day from postonset.On day 16p.i,the rats were killed to gain the brain and spinal cord.The apoptosis condition of spinal cords in rats was detected with in situ end-labeling method.Results：Compared with that of EAE group,apoptosis rate of TUNEL positive cells in E2 group and sham operated group in central nervous system（CNS） were obviously increased.Conclusion：E2 may exert therapeutical effect on EAE.This effect may be related to inhibit the infiltration of the inflammatory cells into CNS and induce apoptosis of this kind of inflammatory cells.