摘要
目的观察微囊藻毒素-LR(MCLR)对人肝癌细胞HepG2的DNA氧化损伤效应。方法应用高效液相色谱-电化学法(HPLC-ECD)和免疫酶染色法检测8-羟基脱氧鸟苷(8-OHdG)水平。结果用HPLC-ECD法分析,低、中剂量组8-OHdG水平显略有增高趋势,高剂量组(100μmol/L)8-OHdG水平显著高于空白对照组;用免疫酶染色法分析,各剂量组8-OHdG染色强度明显高于空白对照组,处理剂量与8-OHdG水平之间呈现剂量-反应关系。结论MCLR引起人肝癌细胞DNA氧化损伤,HPLC-ECD法与免疫酶染色法所测损伤的趋势一致。免疫酶染色法能够更灵敏地检测DNA氧化损伤,可应用于人类疾病研究与环境监测。
Objective To study oxidative DNA damages in human hepatoma cell lines HepG2 treated with MCLR, as determined by different methods. Methods Oxidative DNA damages were determined by high - performance liquid chromatography with electrochemical detection （HPLC -ECD） and immunostaining of 8 - hydroxydeoxiguanosine （8 - OHdG）. Results MCLR at high （100umol/L） doses significantly increased 8 -OHdG levels, and the trend of increase in 8 -OHdG levels was detected in the low and medium doses by HPLC - ECD. The staining intensity of 8 - OHdG in cells treated with different doses of MCLR were stronger than that of untreated cells, and the dose - response relationship was founded by immunosraining of 8 - OHdG. Conclusion MCLR induced oxidative DNA damages in human hepatoma cell lines. The analysis resuits of the two methods are coincidence. Immunostaining of 8 - OHdG is reliable and sensitive method for detecting oxidative DNA damages and could be applied to the study of human diseases and environmental monitoring.
出处
《中国热带医学》
CAS
2008年第9期1505-1506,共2页
China Tropical Medicine
基金
国家留学基金委资助项目(No20845014)
