咳喘宁对支气管哮喘大鼠肺组织NF-κB表达的影响

Effect of Kechuanning on expression of NF-кB in lung tissue of bronchial asthmatic rats

目的:观察咳喘宁对支气管哮喘大鼠肺组织核因子-κB(NF-κB)表达的影响。方法:40只SD大鼠随机分为正常组、模型组、咳喘宁高剂量组(27g生药/kg体质量)、咳喘宁低剂量组(13.5g生药/kg体质量)、桂龙咳喘宁对照组(0.41g/kg体质量),每组8只。除正常组外以卵蛋白致敏并吸入激发法制备大鼠支气管哮喘模型,各治疗组均从第1次哮喘激发开始(造模第3周)至处死前每天灌胃给药,激发并给药4周后处死大鼠,采用免疫组化法观察肺组织NF-κB表达和病理组织学变化。结果:与正常组比较,模型组大鼠支气管管壁和平滑肌厚度、嗜酸性粒细胞、淋巴细胞数以及肺组织NF-κB表达明显增加(P<0.01);与模型组比较,各治疗组均可显著降低支气管管壁和平滑肌厚度、嗜酸性粒细胞、淋巴细胞数以及肺组织NF-κB表达量(P<0.05或P<0.01);且咳喘宁高剂量组优于桂龙咳喘宁组(P<0.05)。结论:咳喘宁可通过抑制NF-κB的表达,降低炎症蛋白基因的转录,减少炎性介质产生,减轻气道炎症。

Objective： To observe effect of Kechuanning on expression of nuclear factor kB （NF-kB） in lung tissue of bronchial asthmatic rats. Methods： 40 rats were randomly divided into 5 groups： the normal group, the model group, Kechuanning high-dose group（27g/kg）, Kechuanning low-dose group（13.5g/kg）, GuilongKechuanning control group（0.45g/kg）, 8 rats in each group. The bronchial asthmatic model was established by egg protein sensibilization and long-term inhalation provocation. The rats of each treatment groups were lavage administration each day from the first time of provocation to execution. After 4 weeks of treatment, the rats were killed and lung tissue were taken to dyeing of HE to be observed. The expression of NF-kB in lung tissue were determined by immunohistochemistry. Result： Compared with the normal group, the thickness of bronchus wall and bronchus smooth muscle, the numbers of eosinophile granulocyte and leukomonocyte, and expression of NF-kB in lung tissue were increased in the model group （P〈0.01）; Compared with the model group, the thickness of bronchus wall and bronchus smooth muscle, the numbers of eosinophile granulocyte and leukomonocyte, the expression of NF-kB in lung tissue were decreased in each treatment group （P〈0.05 or P〈0.01） ,furthermore, the effect of high-dose Kechuanning group were super to that of Guilongekechuanning group （P〈0.05）. Conclusion： Kechuanning could restrain the expression of NF-kB and decrease genic transcription of inflammatory protein to decrease inflammatory preparator and restrain air passage inflammation.