环氧乙烷灭菌营养琼脂培养皿应用的实验观察

Investigation of Agars Culture Mediums Sterilized by Ethylene Epoxide

目的:提出了一种培养皿灭菌方法,即营养琼脂培养皿密封后经过环氧乙烷灭菌,解析后保存备用。方法:通过分析残留环氧乙烷的抑菌影响、不同保存方法对细菌培养的影响,应用不同采样方法空气沉降菌采样,对自然菌捕获率进行了比较。结果:营养琼脂培养皿环氧乙烷灭菌后对空气沉降法或对一些表面的采样,收集细菌的效果和培养结果都与普通9 cm玻璃培养皿相当,其结果具有可比较性。结论:环氧乙烷灭菌营养琼脂培养皿可以在一些现场采样中使用,尤其在突发事件的紧急处理工作中,减少了采样的准备工作,可争取宝贵的时间。

Objective To develop a new method for sterilization in agars culture mediums, i.e. Agars culture mediums is sealed to be sterilized and preserved after resolution. Methods The effect of rudimental Ethylene Epoxide was investigated, and the germieulture results by different preserving methods were analyzed. Air sedimentation bacterium was sampled by different methods to analyze the bacterium capture rate. Results Ethylene Epoxide sterilized agars culture mediums bacterium capture rate was similar with regular agars. Conclusion Ethylene Epoxide sterilized agars culture mediums can be used in sampling of clinical treatment and other outburst incident, reducing the preparation time.