HPLC测定不同产地陈皮橙皮苷的含量

Content Determination of Hesperid in Pericarpium Citri Reticulate of Different Habitats by HPLC

目的：对8批不同产地陈皮药材中橙皮苷的含量进行测定，为科学评价及有效控制其质量提供可靠方法。方法：采用高效液相色谱法，色谱柱为Phenomenex C18色谱柱（250mm×4．60mm，5μm）；流动相：甲醇．冰醋酸-水（35：4：61）；检测波长为283nm；流速：1．0mL·min^-1；柱温：室温；进样量：5μL。结果：橙皮苷在0．102～0．812mg·mL^-1呈良好的线性关系，r=0．9996，平均回收率为98．20％（n=6），RSD=1．22％。不同产地陈皮药材中橙皮苷含量存在差异。结论：该方法简便、快速、准确、重复性好，适用于不同产地陈皮的评价及橙皮苷的含量测定。

Objective： To determine the content of the speridin in eight habitats pericarpium citri reticulate, and gained a method to estimate and control the quality of medical material and effectively. Methods： The HPLC was adopted to do this examination, the separation was performed on a Phenomenex C18 column （250mm × 4. 60mm, 5μm）, methanol-acetic acid glacial-water（35：4：61 ） was used as the mobile phase with isocratic elution, the detective wavelength was at 283nm, the flow rate was 1.0mL-min^-1, the column temperature was room temperature and the injection volume was 5μL. Results： The hesperidin showed a good linearity at the range of 0. 102--0. 812mg·mL^-1, r = 0. 999 6, the average recovery was 98.20%, RSD = 1.22% （n =6） . The contents of hesperidin in pericarpium citri reticulate were varied from different habitats. Conclusion： The method was simple, quick, accurate, good reproducibility, can be used to estimate the pericarpium citri reticulate and determine hesperidin in pericarpium citri reticulate from different habitats.