等离子体聚合沉积聚烯丙胺薄膜改性医用不锈钢及内皮细胞粘附行为研究

Surface modification and endothelial cells adhesion behavior of plasma-polymerized allylamine films deposited on medical stainless steels

采用等离子体聚合沉积方法,通过调控占空比参数,在医用不锈钢表面合成含有伯胺基的聚烯丙胺薄膜,并进一步在聚烯丙胺薄膜表面固定了明胶分子。漫反射傅立叶红外光谱和X射线光电子能谱的分析结果表明聚烯丙胺薄膜表面含有伯胺基团,明胶分子有效地固定到了聚烯丙胺薄膜表面。静态接触角测试结果表明固定了明胶分子的聚烯丙胺薄膜表现出较好的亲水性,具有较高的表面能。体外内皮细胞粘附实验及Alamar Blue评价结果表明固定了明胶分子的聚烯丙胺薄膜表现出良好的促内皮细胞粘附性能,且固定明胶分子的脉冲等离子体聚烯丙胺薄膜表面表现出更高的内皮细胞活性。

In this paper, the polyallylamine films containing primary amine groups on the surface of medical stainless steels were obtained by radio frequency （RF） plasma polymerization by controlling the duty cycle,and the gelatin molecule was further covalently immobilized on the surface of the polyallylamine film. The surface properties of the films were characterized by diffuse reflectance Fourier transform infrared spectroscopy （DR-FTIR）, X-ray photoelectron spectroscopy （XPS） and contacting angle measurement. The analysis of FTIR and XPS showed that polyallylamine films contained primary amine group and the gelatin molecule was effectively immo- bilized on the surface of polyallylamine films. Contacting angle measurement indicated the polyallylamine films immobilized by the gelatin molecule presented better hydrophilicity and higher surface energy. In vitro endothelial cell （EC） adhesion test and Alamar Blue test showed the polyallylamine film immobilized by the gelatin promoted ECs adhesion,and the pulsed plasma-polymerized allylamine film presented better EC activity.