HPLC法同时测定甙原胶囊中儿茶素和表儿茶素的含量

Simultaneous Determination of (+)-Catechin and (-)-Epicatechin in Daiyuan Capsule by HPLC

建立测定甙原胶囊中儿茶素(+)-catechin和表儿茶素(-)-epicatechin含量的方法.采用Kro-masil C18色谱柱(250 mm×4.6 mm,5.0μm),流动相为体积分数为0.1%磷酸水溶液(A)-乙腈(B),进行梯度洗脱;检测波长210 nm,流速1.0 mL/min.结果表明,儿茶素、表儿茶素的线性关系良好,相关系数分别为0.999 4和0.999 6;样品的回收率分别为98.4%和101.2%,RSD分别为0.49%和1.01%(n=9).该法专属性强,结果准确可靠,重复性好,可用于甙原胶囊中儿茶素和表儿茶素的含量测定.

To establish a method of the simultaneous quantative determination of （ ＋ ） -cateehin and （ - ） - epicatechin in Daiyuan Capsnle by HPLC, C18 column was used with the mobile phase of 0.1% phosphoric acid solution and acetonitrile. The detection wavelenth was 210 nm. The linear catlibration curves of （ ＋ ） - catechin and （ - ） -epicateehlnwere obtained in the concentration range ,of 9.41 -21.9 μg ·mL^-1 （ r =0. 999 4） and 9.79-22.8 μg· mL^-1（ r =0.999 6）, respectively. the average recovery of （ ＋ ） - catechin and （-） - epicatechin were 98. 4% ,101.2% and RSD were 0.49% ,1.01%（n =9）. This ,method can simultaneously determinale ,two ingredients in Daiyuan Capsule. The method ,is Simple , sensitive and ：reIiable for quality control.