HPLC法测定小花棘豆中野决明碱含量的研究

Determination of Thermopsine Content in Oxytropis Glabra DC by HPLC

目的建立小花棘豆中主体毒性物质野决明碱含量的HPLC测定方法。方法采用高效液相色谱法，色谱柱：ODS-C18（250mm×4.0mm,5μm）；柱温为30℃.流动相A：甲醛，流速为0.4mL·min^-1；流动相B：0.5％柠檬酸钠＋0.5％磷酸二氢钠，流速为0.6mL·min^-1；检测波长为254mm.结果野决明碱含量与对应的色谱峰面积在0.05～0.04mL·min^-1范围内具有良好的线性关系，r=0.9961;平均回收率为101.91％，精密度为RSD=0.42%（n=5）.结论本方法可有效地用于测定小花棘豆中野决明碱的含量，是一种准确、灵敏、可靠的分析方法。

Objective To establish a method for the determination of the primary poisonous substance - thermopsine in oxytropis glabra DC by HPLC. Methods Analyses were carried out on ODS -C18 （250 mm ×4.0 mm, 5 μm） column. Mobile phase A： methanol, the flow rate 0.4 mL - rain-1 ; mobile phase B： 0.5% sodium citrate ＋ 0.5% sodium dihydrogen phosphate, the flow rate 6 mL · min^-1 The column temperature was 30 ℃. The UV detection wavelength was set at 254 nm. Results The standard curve was linear in the range of 0. 005 - 0.04 mg · mL^-1, the corresponding correlation coefficient r = 0. 9961 ; the averaged recoveries was 101.91% and the precision RSD was 0.42% （n = 5）. Conclusions The method is accurate, sensitive, and reliable. And it could be availably applied to determine thermopsine content in oxytropis glabra DC.