青杨脊虎天牛精子超微结构及其辐照后的变化

SPERM ULTRASTRUCTURE OF Xylotechus rusticus AND THEIR CHANGES AFTER IRRADIATION

研究了青杨脊虎天牛幼虫、蛹和成虫阶段受γ射线辐照后雄虫精子超微结构的变化情况。青杨脊虎天牛雄虫精子分为头部和尾部。头部无顶体,由精核组成,核染色质致密均匀;尾部主要由9+9+2型轴丝和2个在横切面上大小近于相等的线粒体衍生物组成。青杨脊虎天牛成虫、幼虫期和蛹期受γ射线辐照后,雄虫精囊内均发生了2个或多个精子粘连现象。在80Gy剂量辐照下,成虫和幼虫期辐照的精子束内2个精子粘连的现象居多,精子损伤程度基本一致;而蛹期辐照的精子损伤程度略高于成虫和幼虫阶段,精子束内以2个以上精子粘连为主,精细胞内各细胞器排列分散,附体和线粒体衍生物形态改变。120Gy辐照的天牛雄虫精子发生明显肿胀,精核直径为正常精子的10倍多,多个精子粘连的现象增多,精细胞内各细胞器的排列也发生变化,损伤程度明显高于80Gy。

The changes in sperm ultrastructure of male Xylotechus rusticus irradiated by 7-rays on larval, pupal and imaginal stage had been studied. The sperm of male Xylotechus rusticus composed of head and tail. The head with no acrosome, which is completely filled with homogeneous compact chromatin comprises nucleus. The tail consists of an axoneme with the 9 ＋ 9 ＋ 2 microtubule, as well as a pair of mitochondrial derivatives of nearly equal cross sectional size. Sperms in male spermary from irradiated samples have conglutination of two or more sperms when the insects were treated at the stage of adult, larvae and pupae. At the applied dose of 80Gy to adult and larvae, the conglutination of two sperms is predominant in irradiated sperm bundles that of i and the level of irradiation maginal damage is observed identically. However, the irradiation damage was slightly higher than and larval stage when the same dosage was applied to pupae, which shows that the conglutination of much more sperms is predominant in sperms bundle, the scattered arrangement of organelles in spermary, and changes of morphology in accessory bodies and mitochondrial derivatives are also occurred. The male sperms （120Gy） swelled up clearly and nuclear diameter is 10 times of natural sperms, the conglutination of much more sperms is abundant and changes of arrangement of organelles in spermary is also occurred. The degree of irradiation damage at dose of 120Gy is obviously serious than that of 80Gy.