辽河油田冻融石油污染土壤中原位修复微生物

In-situ remediation of microorganisms in frozen and thawed petroleum-contaminated soil from Liaohe Oil Field, Liaoning Province

以辽河油田不同年代开采油井附近采取的土壤样品为研究对象,通过冻融模拟试验和采用选择性培养基筛选对石油污染土壤具有原位修复作用的优势微生物菌株,并比较油田冻结土壤与未冻结土壤中石油降解细菌与真菌的数量变化特征及其对石油的降解能力。结果表明,从未冻结土壤中筛选出石油降解细菌14株、石油降解真菌6株;从冻结土壤中筛选出石油降解细菌5株、石油降解真菌2株。石油降解细菌假单胞菌属(Pseudomonas sp.)的B3号菌株在37℃、7天时间内对石油的降解率最高,为30.2%;石油降解真菌木霉属(Trichoderma sp.)的F3号菌株在28℃、7天时间内对石油的降解率最高,为47.2%。

This paper compares variability of petroleum biodegradation bacteria in frozen and thawed soil collected from Liaohe Oil Field and their capacities for biodegrading petroleum were also determined. The results show that 14 strains of bacteria and 6 strains of fungi for petroleum biodegradation were screened from thawed soils, while only 5 strains bacteria and 2 strains fungi existed in freeze soils. It presents that microorganism diversities in thawed soil is more than that in frozen soil, and that different species of microorganism response to low temperature distinctly. The experiments of petroleum biodegradation indicate that B3 bacterium （Pseudomonas sp.） has the capacity of 30.2 percent to biodegrade petroleum under the condition of 37 centigrade after 7 day＇s incubation. The petroleum biodegradation capacity for F3 fungus （Trichoderma sp. ） is 47.2 percent under the condition of 28 centigrade after 7 days incubation.