原生质体紫外诱变选育纤维素酶高产菌株

Screening and Breeding of Strain Producing High Yield Cellulase through Irradiation of Protoplast by UV

以绿色木霉T0为出发菌株,对其进行原生质体紫外诱变,通过刚果红透明圈平板初筛和油菜籽皮固态发酵复筛,得到一株稳定性好的纤维素酶高产菌株UVT12,其CMC酶活达到了1020.24 U.g-1,比出发菌株T0提高了124.3%。

Using Trichoderma viride To as orginal strain, the irradiation of protoplast by UV was carried out. Through screening＇ by transparent circle on congo red flat at first and then by solid fermentation, a strain of UVT12 producing high yield cellulase with good stability was obtained, and its CMC enzymatic activity reached 1020. 24 U·g^-1,which enhanced 124.3% compared with the start strain T0.