荧光定量RT-PCR检测MCM2 mRNA在结肠癌、结肠腺瘤和正常黏膜中的表达及临床意义

Quantitation of Minichromosome Maintenance 2 mRNA Expression in Carcinoma,Adenoma and Normal Mucosa of Colon with Real-Time RT-PCR Assay

目的探讨细胞增殖标记物微小染色体支持蛋白2(minichromosome maintenance protein 2,MCM2)在结肠癌、结肠腺瘤和正常结肠黏膜中的表达差异及临床意义。方法应用实时荧光定量法检测MCM2 mRNA在12例结肠癌、33例结肠腺瘤和12例正常结肠黏膜中的表达。结果MCM2 mRNA的表达量在正常结肠黏膜、结肠腺瘤和结肠癌组织中依次增高,结肠癌与结肠腺瘤和正常结肠黏膜相比较,MCM2 mRNA的表达上调且差异有统计学意义(P=0.001)。与正常结肠黏膜相比,结肠腺瘤中MCM2 mRNA表达上调,但差异无统计学意义(P=0.184)。结论检测细胞增殖标记物MCM2的表达可评估结肠腺瘤恶变,将有助于结肠癌的早期筛查。

Objective To investigate the difference of minichromosome maintenance protein 2 （MCM2） mRNA expression among colonic normal mucosa, colonic adenoma and carcinoma. Methods The expressions of MCM2 mRNA were determined by real-time RT-PCR in 12 colonic normal mucosa, 33 colonic adenomas and 12 co-lonic carcinomas. Data were evaluated by relative expression software tool （REST-XL）. Results The expressions of MCM2 mRNA elevated in turn by colonic normal mucosa, adenoma and carcinoma. The expression of MCM2 mRNA in colonic carcinomas was significantly higher than that in adenomas, as well as in normal mucosa （P=0.001）, while the difference of MCM2 mRNA expressions between adenoma and normal mucosa was insignificant （P=0.184）. Conclusion The difference of MCM2 mRNA expressions between adenoma and carcinoma indicated the potential value on early diagnosis for colonic carcinoma.