人宫颈癌基因小干扰RNA抑制HepG2细胞生长的作用及其机制

The inhibition effect and its molecular mechanism of human cervical cancer oncogene siRNA on hepatocarcinoma cells

目的利用RNA干扰技术探讨人宫颈癌基因（HCCR）对肝癌细胞增殖、凋亡的影响及其可能的分子机制。方法构建表达HCCR小干扰RNA的真核表达质粒pRIV2-siHCCR，将其转染肝癌细胞株HepG2细胞，定量PCR、Western blot检测HCCR及其相关基因的表达，四甲基偶氮唑盐法检测细胞增殖状态，流式细胞术观察细胞凋亡与细胞周期变化。结果成功构建真核表达质粒pRIV2-siHCCR，其表达的HCCR siRNA可有效抑制HepG2细胞内HCCR的表达，细胞增殖受到抑制，细胞凋亡增加。Western blot结果显示HCCR小干扰RNA作用HepG2细胞后。细胞内P53蛋白的表达降低，P15蛋白的表达明显升高，而PTEN、P16、P27蛋白水平没有明显改变。结论HepG2细胞中HCCR蛋白下调后，细胞增殖受到抑制，凋亡细胞增多，其作用机制可能与蛋白质P53、P15有关。

Objective To evaluate the effect and the possible mechanism ofHCCR siRNA on cell proliferation and apoptosis of hepatocarcinoma cells. Methods pRIV2-siHCCR plasmids, which express small interfering RNA of HCCR were constructed and transfected into HepG2 cells. The mRNA and protein expressions of HCCR were detected by real time PCR and Western blot. The proteins p15, p16, p27, p53, and PTEN were detected by Western blot. The cell proliferation and apoptosis were observed by MTT and FACS. Results The plasmid pRIV2-siHCCR was constructed successfully. Real time PCR and Western blot analysis showed that the HCCR siRNA effectively inhibited HCCR expression in HepG2 cells after pRIV2-siHCCR transfection. MTT method confirmed that HepG2 cell proliferation was suspended, while the cell apoptosis was increased much more than that in the control group. After the transfection with the plasmid of pRIV2- siHCCR into HepG2 cells, the expression of p53 protein was decreased, and P15 increased; and levels of PTEN, p16, and p27 were evidently not changed. Conclusion After being transfected with HCCR siRNA expression plasmid, the cell proliferation of HepG2 was arrested, while the apoptosis of HepG2 cells increased. Our results demonstrate the potential role of p53 and p15 in HCCR signaling.