摘要
将人α-乳清白蛋白基因构建到植物表达载体上,通过农杆菌介导采用叶盘法将人α-乳清白蛋白基因导入到烟草中,经过PCR和Southern blot分析表明:人α-乳清白蛋白基因已经整合到烟草基因组中;经过RT-PCR和GUS组织化学染色证明:人α-乳清白蛋白基因得到了表达。测定了9株转基因烟草叶片半胱氨酸含量,大部分植株有着明显的提高,最高幅度达到了318.02%,半胱氨酸含量平均提高了166.40%。
The plant expression vector containing human alpha-lactalbumin gene was constructed and its gene was transformed into tobacco using leaf disc mediated by Agrobacterium tumefaciens LBA 4404 as a vehicle. PCR and Southern blot analyses showed that human alpha-lactalbumin gene was integrated into the tobacco genome. RT-PCR and histochemical staining for GUS activity demonstrated that human alpha-lactalbumin gene was expressed in transgenic tobacco plants. Further analysis of the data indicated that the average of cysteine contents in 9 transgenic tobacco plants was greatly increased by 166.40% besides one of them which was increased by 318.02%.
出处
《云南植物研究》
CSCD
北大核心
2008年第4期457-463,共7页
Acta Botanica Yunnanica
基金
the scientific and technological program of Fujian Science and Technology Department(2004N026)
the Natural Science Foundation of Fujian Province(B0410009)
the scientific and technological program of undergraduate students in Fujian Normal university(BKL2007062)
