手性双核钌(Ⅱ)配合物与DNA的相互作用研究

Interaction between DNA and Chiral Dinuclear Ru(Ⅱ) Complexes

本文合成了1对手性双核钌(Ⅱ)配合物ΔΔ-和ΛΛ-[(bpy)2Ru(mbpibH2)Ru(bpy)2](ClO4)4(bpy=2,2′-联吡啶,mbpibH2=1,3-二(咪唑并[4,5-f][1,10-邻菲咯啉])苯)。通过元素分析、质谱、核磁共振、CD光谱对这两个化合物进行了结构表征。采用循环伏安法对配合物的电化学性质进行了分析。利用紫外-可见吸收光谱滴定、荧光光谱滴定、稳态荧光淬灭和粘度实验研究了配合物与DNA的相互作用,实验结果表明这2个配合物都能以插入的方式与DNA键合,而且ΔΔ配合物的DNA结合强度稍强于ΛΛ配合物。

The enantiomerically-pure chiral dinuclear Ru（Ⅱ） complexes AA_ and AA-[（bpy）2Ru（mbpibH2）Ru（bpy）2] （ClO4）4 （bpy=2,2＇-bipyridine, mbpibH2= 1,3-bis（[ 1,10]phenanthroline-[5,6-d]imidazol-2-yl）benzene） were synthesized and characterizated by elemental analysis, MS, 1H NMR and CD spectra. The electrochemical behaviors of the complexes have been studied by cyclic voltammetry. Binding of the complexes with CT-DNA has been investigated by absorption titration, luminescence titration, steady-state emission quenching and viscosity experiments. The results indicate that both the enantiomers can intercalate into CT-DNA while the binding ability of △△ is stronger than that of ∧∧.