扁玉螺体表和消化系统甲硫氨酸脑啡肽免疫组织化学定位

Immunohistochemical Localization of Methionine-enkephalin in the Body Surface and Digestive System of Neverita didyma

采用免疫组织化学strept avidin-biotin complex(S-ABC)法对甲硫氨酸脑啡肽(methionine-enkephalin,M-ENK)在扁玉螺(Neverita didyma)体表、消化系统各器官中的分布进行了研究。结果表明,扁玉螺的足上皮细胞、外套膜的内外上皮细胞以及食道、胃、肠道的黏膜上皮细胞均呈M-ENK阳性反应,且消化道中的阳性反应多集中于上皮细胞游离端;在食道腺的腺上皮中也有少量阳性细胞分布;肝是M-ENK阳性细胞分布较多的器官,主要分布在肝小叶中腺细胞边缘游离端。M-ENK在扁玉螺体表和消化系统各器官均有分布,且分布密度有所不同,可能与各部位的功能有关。

The methionine-enkephalin（M-ENK）in the body surface and digestive system of Neverita didyma was analyzed by the immunohistochemical S-ABC （strept avidin-biotin complex） staining. The results showed that M-ENK positive cells existed widely in the epithelium of foot, inner and outer epithelia of mantle, mucous membrane epithelium of oesophagus, stomach and intestine. Besides, the positive immuno-reaction was centralized in the dissociative sides of alimentary canal epithelium. A small quantity of positive cells was observed in the esophageal gland, too. A great number of positive cells were observed in the liver, mostly in the dissociative sides of the gland cells. It can be concluded that M-ENK is abundantly distributed in the epidermis of the body surface and digestive system in N. didyma, and that different densities of M-ENK in these organs may be referable to their different functions.