EB病毒感染和p16^INK4a蛋白过表达与胃腺癌的关系

Epstein-Barr virus infection and p16^INK4a overexpression in gastric adenocarcinoma

目的探讨Epstein-Barr Virus（EBV）感染与p16异常表达在胃腺癌发生发展中的作用。方法应用检测免疫组化法检测EB病毒潜伏感染膜蛋白-1（LMP-1）和p16蛋白在97份胃腺癌组织中表达。结果97份胃腺癌组织中30例LMP-1蛋白表达阳性，阳性率为30．9％，EBV阳性率与患者性别、浸润深度、淋巴结转移、组织学分型和临床分期之间无明显关系（P〉0．05）；p16蛋白在胃腺癌组织中过度表达，阳性率为63．91％，p16过度表达与患者性别、肿瘤组织学类型、浸润深度不相关．而与淋巴结转移和临床分期相关；EBV感染与p16阳性表达之间无显著相关性（P〉0．05）。结论①EBV感染在胃腺癌的发生中起一定作用；②胃腺癌组织中p16蛋白过度表达是频发事件，p16是重要的预后指标之一；③EBV感染与p16异常表达在胃腺癌发生中是两个独立因素。

Objective To study Epstein-Barr virus infection and p16 protein abnormal expresson in carcinogenesis and progression of gastric adenocarcinomas （GAC）. Methods Immunohistochemical staining SP method was used to detect the expression of LMP-1 and p16 in 97 cases of GAC. Results EBV LMP-1 and p16 protein were detected in 30.9% （30/97） and in 63.91% （62/97） cases of gastric ade respectively There was no significant difference between EBV-positive and EBV-negative gastric carcinomas in sex, histologic type, depth of tumor invision, lymph node metastasis and clinical stages （ P 〉 0.05） ; overexpression of p16 was associated with lymph node metastasis and clinical stages ; no correlation was found between the expression of EBV LMP-1 and p16 protein. Conclusion ①EBV play a role in carcinogensis of GAC. ②P16 gene abnormality is frequently involved in GAC and might be one of the important prognostic factors. ③ EBV infection and p16 alteration are two independent roles in GAC carcinogenesis.