应用聚合酶链反应检测食管癌组织中人乳头瘤病毒

Detection of human papillomavirus in tissues of esophageal carcinomas by polymerase chain reaction

目的探讨人乳头瘤病毒（human papillomavirus，HPV）与我国河南地区食管癌发生的相关性。方法应用HPVL1通用引物GP5＋／6＋、HPV16E6和HPV18E6型特异性引物多聚酶链反应（Pol，merase chain reaction，PCR），检测林州市食管癌组织中HPV存在状况。结果3l例食管癌组织中，29例检测到HPV阳性，阳性率为93．5％；其中19例检测到HPV16E6基因，阳性率为61．3％，8例为HPV18E6基因阳性，阳性率为25．8％，5例HPV16E6和18E6基因均阳性，为混合感染。HPV16和18型阳性率为71．0％。结论我国河南省林州市食管癌组织中有HPV存在，并且HPV感染可能是食管癌发生的重要病因。

Objective To investigate the relationship between the human papillomavirus （HPV） and esophageal carcinomas. Methods We detected HPV DNA in 31 fresh tissue of esophageal carcinomas from Linzhou City, Henan Province, by PCR with the general primer set of GP5＋/6 ＋ for HPV L1 gene and typespecific primer sets for HPV16 and 18 as well. Results 29 from 31 esophageal carcinoma samples were HPV positive and the rate was 93.5% . Among the samples detected, 19 were HPV16E6 positive and rate was 61.3% , eight were HPV18 E6 positive and rate was 25.8%; our result also showed five were the multiple infection containing HPV16 and 18 as well and the rate was 71.0% . Conclusion The high rate of HPV in the esophageal carcinoma samples suggested that HPV plays an etiologic role in the development of esophageal cancer in Linzhou City, Henan Province.