维百氏康空气健康剂抗流感病毒初步研究

Primary research of anti-influenza virus activity of ＂BenovoairTM Concentrate＂

目的进行维百氏康空气健康剂抗流感病毒实验研究方法将不同稀释度的样品与100TCID50，的病毒液等量混合，37℃中和30min，取适量接种于含维持液的细胞孔内，一式3孔，设病毒对照、细胞对照和不同稀释度样品对照，置37℃CO2培养箱，逐日观察CPE，当病毒对照组CPE出现＋＋＋＋时终止试验，测定血凝程度。结果样品原液的1：60，1：120，1：240，1：480可100％灭活甲型及乙型流感病毒。原液的1：960和1：1920可灭活25％～50％甲型及乙型流感病毒。结论为维百氏康空气健康剂产品的研发提供了抗流感病毒试验依据。

Objective Anti-influenza virus activity of ＂Benovoair^TM Concentrate＂. Methods The different dilution of samples were mixed with the same quantity of 100 TCID50 virus at 37℃ for 30 minutes. Add suitable quantity mixture in wells containing cells. Every 3 wells were the same mode. Viruses control, cells control and samples control of different dilution were pe.rformed and set in the CO2 incubator at 37℃ . CPE was observed every day. When CPE appears in viruses control as ＂ ＋＋＋＋ ＂, stopped testing and performed the hemagglutination titration. Results ＂BenovoairTM Concentrate＂ with dilution of 1 60, 1 120, 1 240 and 1 480 have 100% antiinfluenza A and anti-influenza B activities. ＂Benovoalr＂ ^TM Concentrate＂ with dilution of 1 960 and 1 1920 have 25%-50% anti-influenza A and anti-influenza B activities. Conclusion The test was the proof of anti-influenza virus activities which provided for the development of ＂BenovoairTM Concentrate＂.