TAT-HBX-EGFP融合蛋白的表达、纯化及在小鼠肝脏内的跨膜分布

Expression and purification of TAT-HBX-EGFP fusion protein and its transmembrane distribution in mouse liver

目的高效表达TAT—HBX—EGFP融合蛋白，研究其在小鼠肝脏内的分布。方法构建TAT-HBX—EGFP重组载体，IPTG诱导使其在BL21中大量表达；蛋白经Ni柱纯化后注射入小鼠体内，免疫荧光观察TAT，HBX—EGFP融合蛋白在小鼠肝脏的分布。结果TAT，HBX—EGFP在大肠埃希菌中获得高效表达；HBX蛋白可以在TAT的引导下进入小鼠肝脏。结论TAT引导肽可以将HBX蛋白引导至小鼠肝脏。

Objective To highly express TAT-HBX-EGFP fusion protein and study its distribution in mouse liver. Methods TAT-HBX-EGFP recombinant vector was constructed and fusion protein was induced by IPTG and expression in BI221 ; fusion protein was purified by Ni-NTA argarose, then injected into the peritoneal cavity of the mice. Distribution of fusion protein was observed by immunofluoreseence. Results TAT-HBX-EGFP was highly expression in E. coli; HBX could be induced into mouse liver by TAT. Conclusion HBX protein could be induced into mouse liver by TAT induced peptide.