慢性乙型肝炎患者血清趋化因子RANTES水平的检测及意义

The measurement and significance of serum chemokine RANTES in patients with chronic hepatitis B

目的了解慢性乙型肝炎患者血清趋化因子RANTES水平，探讨不同病情慢性乙型肝炎患者血清RANTES水平变化情况及原因分析。方法选择144例慢性乙型肝炎患者，按照病情分为轻中度组（46例）、重度组（51例）及重型肝炎组（47例）以及18名健康人作为正常对照组，应用ABC—ELISA方法检测患者及正常健康人血清中趋化因子RANTES浓度，利用SPSS13．0软件进行各组之间统计分析。结果慢性乙型肝炎患者血清中RANTES的浓度均比正常对照组增高，且随着慢性乙型肝炎患者病情的加重，血清中RANTES浓度逐渐升高，但重型肝炎组较重度肝炎组下降，正常对照组、轻中度肝炎组、重度肝炎组及重型肝炎组患者血清RANTES浓度分别为（29．46±152．00）ng／L、（2227．06±790．80）ng／L、（5878．49±3334．58）ng／L和（3482．77±2315．62）ng／L，各组之间差异比较均有统计学意义（P〈0．05）。结论慢性乙型肝炎患者血清中RANTES表达水平增高，趋化因子RANTES可能参与慢性乙型肝炎的发病。

Objective To investigate the relationship of the serum chemokine RANTES level in patients with chronic hepatitis B among different clinical severity and to explore its possible reasons. Methods 144 patients with chronic hepatitis B were divided into mild-moderate （46）, serious（51 ） or severe group（47 ） according to the different clinical severity and 18 normal cases were taken as normal control. The serum level of chemokine RANTES was detected with an ABC-ELISA assay. Statistical analysis was performed on the software of SPSS 13.0. Results The serum chemokine RANTES levels in patients with chronic hepatitis B （2227.06 ± 790.80, 5878.49 ±3334.58, 3482.77± 2315.62 ng/L in mild-moderate, serious and severe group respectively） were significantly higher than that in the normal control （329.46 ± 152.00 ng/L）. The differences between each two hepatitis groups were also statistically significant （P 〈 0.05） and serum chemokine RANTES level in serious group was highest among them. Conclusion Serum chemokine RANTES level in patients with chronic hepatitis B elevates significantly and it might play an important role in the pathogenesis of chronic hepatitis B.