冬眠合剂对严重烫伤大鼠肺损伤的保护作用

Protective effect of lytic cocktail （inhibition of stress） on lung injury in severe burn rat

目的观察早期应用冬眠合剂对严重烫伤大鼠肺损伤是否具有保护作用。方法建立Ⅲ度烫伤大鼠模型，随机分为冬眠合剂组和对照组（未用冬眠合剂），每组36只。分别在伤后3、5、7、10d测定大鼠动脉血氧分压，检测肺组织中过氧化物酶（MPO）活性、丙二醛（MDA）含量、肿瘤坏死因子α（TNF—α）及γ干扰素（IFN-γ）蛋白表达，动态观察伤后肺组织病理学变化。结果与对照组大鼠伤后3d动脉血氧分压（8．86±0．23）kPa（1kPa=7．5mmHg）比较，冬眠合剂组该时相点明显升高[（12．58±0．41）kPa，P〈0．01]。冬眠合剂组大鼠伤后各时相点肺组织MPO活性和MDA含量下降（P〈0．05或P〈0．01）；伤后3、5、7d肺组织TNF—α表达显著下调（P〈0．05或P〈0．01），伤后5、7、10d肺组织IFN-γ的表达亦显著下调（P〈0．01）。冬眠合剂组大鼠肺泡间质水肿减轻，炎性细胞浸润减少。结论大鼠烫伤后及时复苏并给予冬眠合剂，能适度抑制机体应激反应并下调促炎因子表达，改善早期肺功能。

Objective To investigate the protective effect of inhibition of stress （ lyric cocktail） on lung injury in severe burn rats at early stage. Methods Sprague-Dawley rats inflicted with 30% TBSA fullthickness burn were randomly divided into A group （ n = 36, fluid resuscitation with administration of lyric cocktail） , B group（ n = 36, fluid resuscitation only）. Lung function was evaluated by partial pressure of ox ygen （PaO2 ） in arterial blood and histopathologic changes on 3, 5, 7, 10 post burn day （PBD）. The levels of malonyldialdehyde （ MDA）, myeloperoxidase （ MPO）, tumor necrosis factor-α（TNF-α） and interferon-γ （IFN-γ） in lung tissue were measured at the same time points. Results The PaO2 level in A group on 3 PBD （12.58±0.41 kPa） was significantly higher than that in B group （8.86 ±0.23 kPa, P 〈0.01）. Compared with those in B group, the levels of MDA and MPO were significantly decreased in A group at each time point（ P 〈0.05 or0.01） ,the levels of TNF-α on 3,5,7 PBD （ P 〈0.05 or0.01）and IFN-γ on 5,7,14 PBD （ P 〈 0.01 ） were also decreased in A group. Swollen lung mesenchyme was alleviated,infiltration of inflammatory cell was lessened in A group. Conclusion Lyric cocktail combined with immediate fluid resusitation can inhibit stess response, downregulate the expression of inflammatory factor,ameliorate lung function in severe burn rat at early stage.