混合脐血血浆对植物血凝集素诱导的脐血源性T淋巴细胞增殖、分化的影响

Effects of mixed cord blood plasma on proliferation and differentiation of PHA-induced cord blood-derived T lymphocyte

目的:探讨混合脐血血浆(CBP)在植物血凝集素(PHA)诱导脐血源性T淋巴细胞中的作用。方法:收集脐血血浆和脐血单个核细胞;将脐血单个核细胞分为CBP+PHA和PHA两组,检测两组细胞增殖率及免疫表型。结果:CBP+PHA组细胞增殖指数低于PHA组,但无统计学意义;两组细胞表型都以CD3+、CD4+为主,两组差异无统计学意义。结论:混合脐血血浆对脐血源性T淋巴细胞增殖、分化影响不明显,可能与RPMI1640培养体系具有相似的效果。

Objective： To explore the effects of mixed cord blood plasma （CBP） on phytohemagglutin （PHA） induction of cord blood-derived T cells. Methods： Cord blood plasma and umbilical cord blood mononuclear cells （CBMCs） were collected; CBMCs were divided two groups： CBP＋ PHA group and PHA group, and the proliferation index and immune pheno- types of each group were detected. Results： The proliferation index of CBP＋PHA group was lower than that in the PHA group without significant difference （P〉0. 05） ; the immune phenotypes of two groups were both mainly CD3- and CD4 without significant difference （P〉0. 05）. Conclusion： The effects of CBP on proliferation and differentiation of cord bloodderived T cells were similar to those of 20% RPMI1640.