摘要
目的:探讨混合脐血血浆(CBP)在植物血凝集素(PHA)诱导脐血源性T淋巴细胞中的作用。方法:收集脐血血浆和脐血单个核细胞;将脐血单个核细胞分为CBP+PHA和PHA两组,检测两组细胞增殖率及免疫表型。结果:CBP+PHA组细胞增殖指数低于PHA组,但无统计学意义;两组细胞表型都以CD3+、CD4+为主,两组差异无统计学意义。结论:混合脐血血浆对脐血源性T淋巴细胞增殖、分化影响不明显,可能与RPMI1640培养体系具有相似的效果。
Objective: To explore the effects of mixed cord blood plasma (CBP) on phytohemagglutin (PHA) induction of cord blood-derived T cells. Methods: Cord blood plasma and umbilical cord blood mononuclear cells (CBMCs) were collected; CBMCs were divided two groups: CBP+ PHA group and PHA group, and the proliferation index and immune pheno- types of each group were detected. Results: The proliferation index of CBP+PHA group was lower than that in the PHA group without significant difference (P〉0. 05) ; the immune phenotypes of two groups were both mainly CD3- and CD4 without significant difference (P〉0. 05). Conclusion: The effects of CBP on proliferation and differentiation of cord bloodderived T cells were similar to those of 20% RPMI1640.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2008年第4期515-518,共4页
Chinese Journal of Anatomy
关键词
脐血血浆
T淋巴细胞
增殖
分化
cord blood plasma
T lymphocyte
proliferation
differentiation