慢性疲劳应激对大鼠伏隔核一氧化氮合酶表达的影响

Effects of chronic fatigue on the expression of nitric oxide synthase in rat nucleus accumbens

目的:探讨长时间游泳引起的疲劳应激对大鼠伏隔核(NAc)中神经元型一氧化氮合酶(nNOS)表达的影响。方法:采用成年雄性大鼠连续4周每天强迫游泳至力竭,用免疫组织化学方法结合图像处理测定伏隔核中nNOS的表达。结果:长时间强迫游泳应激可使大鼠伏隔核中nNOS免疫细胞化学反应阳性神经元的数量(106.7%)、面积(150.2%)和灰度值(11.3%)显著增加。结论:nNOS参与疲劳应激的形成,一氧化氮在伏隔核对应激调节中起重要作用。

Objective： To assess the effects of chronic fatigue induced by swimming on the expression of neuronal nitric oxide synthase （nNOS） in the rat nucleus accumbens. Methods： Adult male Wistar rats were forced to swim till exhaustion every day for 4 weeks, Immunohistochemistry combined with the image manipulation was used for the measurement of nNOS positive neurons. Results： A long-time exposure to chronic forced swimming significantly increased the number （ 106.7 ~ ）, dis- tributed area （150. 2~） and gray degree （11.3%） of nNOS immunoreactive positive neurons in the nucleus accumbens. Conclusion ： nNOS might be involved in the formation of fatigue stress, and that nitric oxide （NO） might play an important role in the regulation of stress in the nucleus accumbens.