血管抑素基因和内皮抑素基因抑制肝癌作用的比较

Comparative study on inhibitory effects of angiostatin gene and endostatin gene on growth of hepatoma

目的:比较血管抑素(angiostatin)和内皮抑素(endostain)基因抑制肝癌作用的差异。方法:建立大鼠肝癌模型,随机分组,在肝癌局部分别注入血管抑素基因、内皮抑素基因、血管抑素+内皮抑素基因及0.9%氯化钠溶液,观察各组肿瘤微血管密度(microvessel density,MVD)、肿瘤凋亡率、肺转移瘤的数量、大鼠生存期及肿瘤生长率等指标。结果:所有治疗组的肿瘤体积生长率均受到抑制,以双基因联合的作用最为明显。各治疗组肺内转移灶数、MVD和凋亡指数与对照组相比,差异均有统计学意义(P<0.01,P<0.05和P<0.05);双基因联合应用组与其他治疗组相比,差异具有统计学意义(P<0.05);而单一基因治疗组之间比较差异无统计学意义(P>0.05)。双基因联合应用后大鼠存活时间最长,而对照组生存时间最短。结论:血管抑素、内皮抑素均通过抑制肿瘤新生血管的生长、迁移而达到抑制肿瘤生长和转移的目的,2种抑素基因的协同作用更加明显。

Objective：To make a comparative study on therapeutic action of angiostatin gene and endostatin gene on hepatoma. Methods：The xenografted hepatoma models were established in rats. The tumor-bearing rats were randomly divided into four groups. Each group were locally injected 200 μL of endostation gene, angiostatin gene, endostation gene plus angiostatin gene, and 0.9% NaCl solution, respectively. The microvessel density （ MVD）, tumor apoptosis rate, the number of pulmonary metastasis, survival time, and tumor growth rate were recorded. Results：The tumor growth was inhibited in all the therapeutic groups. The inhibitory action was most significant in the double gene therapy group. There was significant difference in the number of pulmonary metastasis, MVD, apoptosis index （AI） between the therapeutic groups and control group （P 〈0.01, P 〈0.05, and P 〈0.05 ）. The difference was statistically significant between the double gene therapy group and single gene therapy group （ P 〈 0.05 ）. However, there was no significant difference between endostatin group and angiostatin group （P 〉 0.05）. The survival time of rats was the longest in the double gene therapy group and the shortest in the control group. Conclusion：Endostatin and angiostatin inhibit the tumor growth and metastasis by repressing tumor angiogenesis and migration. Both statins have synergistic action in inhibiting tumor growth.