骨桥蛋白和整合素αⅤ表达与喉和下咽鳞状细胞癌侵袭转移的相关性

The expression of osteopontin and integrin αⅤ in the laryngeal and hypopharyngeal squamous cell carcinoma and its correlation with tumor invasion as well as metastasis

目的:探讨骨桥蛋白(osteopontin,OPN)及其受体整合素αⅤ(integrin αⅤ,ITGAⅤ)在喉和下咽鳞状细胞癌(laryngeal and hypopharyngeal squamous cell carcinoma,LHSCC)中的表达及其对肿瘤侵袭转移等生物学行为的影响。方法:设计并制作喉和下咽鳞状细胞癌组织芯片,应用免疫组织化学技术在组织芯片上检测OPN和ITGAⅤ蛋白表达,应用专业级病理图像处理软件(Image Pro Plus 5.02,IPP 5.02)对免疫组织化学染色结果进行相对定量,分析OPN和ITGAⅤ的表达量与喉和下咽鳞状细胞癌临床病理分期的相关性。结果:原发癌及转移癌中OPN和ITGAⅤ的表达量均明显高于正常黏膜,差异有统计学意义(P<0.001);高分化组的OPN和ITGAⅤ表达量明显低于中分化组和低分化组,差异均有统计学意义(P<0.001);有淋巴结转移组的OPN和ITGAⅤ表达量明显高于无淋巴结转移组(P<0.001);2者的表达量与肿瘤的原发部位、浸润范围以及患者的年龄、性别等因素均无相关性。结论:OPN和ITGAV的表达与喉和下咽鳞状细胞癌的侵袭转移相关,OPN和ITGAⅤ的过表达可能是促成喉癌和下咽癌侵袭转移的重要因素之一。

Objective： To investigate the expression of osteopontin （OPN） and integrin α V （ ITGA V ） in the laryngeal and hypopharyngeal squamous cell carcinoma （LHSCC） and study the influence on the biological behaviors （invasion and migration） of tumor cells. Methods： The tissue microarray of LHSCC was designed and the OPN and ITGAV expressions were detected by immunohistochemistry. The professional software of pathological image manipulation （ Image Pro Plus 5.02, IPP 5.02） was used to quantitate the resuits of the immunohistochemical staining. The relationship between OPN and ITGAV expressions and elinicopathological staging of LH- SCC was analyzed. Results： The expression levels of OPN and ITGAV in primary and metastatic carcinoma were significantly higher than those in normal tissues （P 〈 0. 001 ） ; their expressions in the well-differentiated group were significantly lower than those in the moderate- and poor-differentiated groups（ P 〈0.01 ） ;the expression levels of 0PN and ITGAV in the group with lymph node metastasis were significantly higher than those in the group without lymph node metastasis （P 〈 0. 001 ）. The expression levels of OPN and ITGAV had no correlation with primary locations, the size of invasion area, and the age and sex of patients. Conclusion： The expressions of OPN and ITGAV are significantly related with the invasion and migration of the LHSCC. Over-expression of OPN and ITGAV may be one of the important factors contributing to the invasion and migration of LHSCC.