选择性环氧合酶-2抑制剂NS-398体外抑制结肠癌细胞增殖和侵袭

Inhibitory effect of selective cyclooxygenase-2 inhibitor NS-398 on proliferation and invasion of human colorectal cancer cells

目的:观察环氧合酶-2(COX-2)抑制剂NS-398对结肠癌细胞增殖、侵袭的影响,探讨COX-2作为结肠癌治疗靶标的可行性。方法:用RT-PCR和Western印迹法检测结肠癌细胞CW-2、COLO-320中COX-2基因及蛋白的表达。MTT法观察NS-398对COLO-320细胞增殖的抑制作用;体外细胞侵袭试验检测NS-398对细胞侵袭能力的影响;West-ern印迹检测NS-398对基质金属蛋白酶2(MMP-2)表达的影响。结果:结肠癌细胞株CW-2、COLO-320中COX-2均有阳性表达。NS-398能抑制结肠癌细胞株COLO-320的生长,抑制作用具有时间、浓度依赖性。细胞侵袭试验表明,NS-398体外能抑制结肠癌细胞株COLO-320的侵袭。Western印迹结果表明NS-398可以抑制COLO-320中MMP-2的表达。结论:COX-2抑制剂NS-398体外可显著抑制结肠癌细胞的生长、侵袭,COX-2可作为结肠癌治疗的靶点。

Objective： To investigate the effect of NS-398, a selective cyclooxygenase-2 （COX 2 ） inhibitor, on the proliferation and invasion of human colon carcinoma cells in vitro ,so as to determine the possibility of COX-2 as a new target for treatment of colon carcinoma. Methods： The expression of COX-2 in colorectal cancer cells （CW 2,COLO-320） was detected by RT PCR and Western blotting. COLO-320 cell proliferation was measured by MTT after treatment with NS-398. Cell invasion ability was measured using migration and invasion chamber systems. Western blotting assay was used to examine the influence of NS-398 on MMP-2 expression. Results： Our results showed that CW-2, COLO-320 cells expressed COX-2 mRNA and protein. NS398 inhibited the proliferation of COLO-320 cells in a time- and concentration-dependent manner. Invasion test showed that NS-398 inhibited the migration and invasion of COLO-320 cells. Western blotting revealed that NS-398 inhibited the expression of MMP-2 in COLO-320 cells. Conclusion： The selective COX 2 inhibitor NS-398 can inhibit COLO-320 cell proliferation and invasion,indicating COX-2 may serve as a new target for colon carcinoma treatment.