缺血预处理对肝脏缺血再灌注损伤的保护机理探讨

lschemic Precondition Protects the Liver from Damage by Ischemia-reperfusion

目的研究缺血预处理对肝脏缺血再灌注损伤的保护机制。方法将健康大鼠随机分为3组:假手术组、缺血再灌注组和缺血预处理组。测定各组大鼠的ALT、AST和LDH,同时用TUNEL法测定肝细胞的凋亡,使用免疫组化法对凋亡相关基因bcl-2和bax进行检测。结果缺血60 min和再灌注120 min后,与缺血再灌注组相比,缺血预处理组的肝功能损伤较轻,凋亡细胞减少,bcl-2表达较多,bax表达减少。结论肝脏缺血预处理通过提高抗凋亡基因的表达,减少促凋亡细胞,降低了肝细胞的凋亡率,预防缺血再灌注对肝细胞的损伤。

Objective To investigate how the ischemic precondition protects the liver from ischemiareperfusion. Methods The healthy rats were divided into three groups： sham-operation group,ischemiareperfusion group and ischemic precondition group. The ALT, AST and LDH of the rats in these groups were tested. TUNEL was applied to measure the hepatocyte apoptosis, and immunohistochemistry was to determine the apoptosis associated genes, bcl-2 and bax. Results Afterischemia for 60 minutes and reperfusion for 120 minutes, the liver function in the precondition group was much better than that in the ischemia-reperfusion group. Less apoptosis andmore bcl-2 expression and less bax expression were observed in precondition group. Conclusion The precondition protects liver from chemia-reperfusion by promoting the anti-apoptosis gene expression, decreasing the apoptosis gene expression and reducing the hepatocyte apoptosis.