Cox-2蛋白在宫颈癌中的表达及与细胞凋亡的关系

The expression of Cox-2 protein and its relationship to apoptosis in cervical carcinoma

目的:研究Cox-2蛋白在宫颈癌放疗及放化疗过程中的表达及其与肿瘤细胞凋亡的关系,以指导临床治疗。方法:60例患者随机分成2组,单纯放疗(radiotherapy RT)组30例给予10 Gy放疗,放化疗(synchronal radiochemotherapy CRT)组30例先行一次化疗,再给予10 Gy放疗,两组放疗前后均取活检,采用免疫组织化学法和脱氧核糖核酸转移酶介导的缺口末端标记(TUNEL)技术,分别检测30例单纯放疗和30例放化疗联合宫颈癌患者治疗前后宫颈肿瘤细胞凋亡率及Cox-2蛋白的表达。结果:在RT组和CRT组中治疗前Cox-2蛋白的表达分别为50%和53.33%,治疗后分别为30%和23.33%,表达阳性率治疗后较治疗前都有明显降低,CRT组治疗前后阳性率有统计学差异(P<0.05),但RT组治疗前后阳性率对比无统计学差异(P>0.05)。RT组放疗前和放疗10 Gy后,肿瘤细胞凋亡率分别为33.33%和66.67%,有统计学差异(P<0.05);CRT组治疗前和先行一次化疗再放疗10 Gy后,肿瘤细胞凋亡率分别为36.67%和93.33%,两者有统计学差异(P<0.01)。治疗后组间对比,放化疗组凋亡阳性率明显高于单纯放疗组,差异有统计学意义(P<0.05)。相关分析表明,肿瘤细胞中治疗前及治疗后细胞凋亡与Cox-2表达均呈显著等级负相关。结论:Cox-2可能通过调控肿瘤细胞的凋亡发挥作用,并在肿瘤恶性转化中可能起着重要作用。因此,检测Cox-2蛋白的表达有可能作为判断宫颈癌的生物学行为和临床预后的指标之一。

Objective： To study of the the expression of Cox-2 and its relationships to apoptosis in cervical carcinoma （UCC） during radiochemotherapy. Methods： Sixty patients with UCC were divided randomly into two groups： the radiotherapy （RT） group with 30 patients who were given simple external irradiation of the pelvic cavity and after-loading therapy and, the synchronal radiochemotherapy （CRT） group with 30 patients who were given one cycles of chemotherapy besides the RT. Biopsy of the UCC was conducted be fore and during the treatment （group RT： after 10 Gy radiotherapy; group CRT： 10 Gy radiotherapy; chemotherapy one cycle）. The samples obtained in the treatment were employed to determine apoptosis and the expression of Cox-2 protein using TUNEL and immunohistochemical methods. Results： Before and during the treatment, The positive rate of Cox2 protein reduced. There were 50% and 53.33% before treatment, and 30% and 23.33% after treatment, in the CRT group with a significant difference （P 〈0. 05）. However, in the RT group with no significant difference （P 〉0.05）. Before and during the treatment, the positive rates of apoptosis were increased, ranging from 33.33%to 66.67% （P 〈0.05） in the RT group and from 36.67% to 93.33% （P 〈0.05） in the CRT group, respectively. There was a significant difference between the two groups. However, There was a significant correlation between the expression of apoptosis and Cox-2 after treatment in both groups. Conclusion： Cox2 plays an important role in cervix carcinoma development by control apoptosis of the tumor cells. To detect the expression of Cox -2 can be one of the maker of biological behavior and clinical prognosis in uteri cervix carcinoma.